Escherichia coli Strain Collection

EcL Laboratory’s Escherichia coli strain collection includes more than 10,000 E. coli strains isolated since 1978 from clinical cases of different animal species, particularly pigs.  Since 2017, more than 1,000 strains have been sequenced by whole genome sequencing (WGS), allowing us to obtain profiles of virulence genes, antibiotic resistance genes, ST (MLST), phylogroup and O:H serotype, thus completing the information previously obtained by PCR or agglutination.

To view the full EcL Laboratory website, click here

 

Animal species of origin:

Swine, Cattle, Avian (Chicken, Turkey, Duck), Ovine, Goat, Equine, Rabbit, Dog, Cat, Wild animals, Exotic animals.

 

Organs:

Ileum, colon, feces, liver, lung, heart, pericardium, yolk sac, air sac etc.

Observed clinical signs or disease:

Diarrhea, sudden death, edema disease (swine), hemorrhagic dysentery (cattle), colisepticemia (avian), aerosacculitis (avian), urinary tract disease (dog, cat), sepsis, omphalitis (avian), paralysing gastroenteritis (cattle), granulomatous colitis (dog), presence in extraintestinal organs.

Available pathotypes:

 

-Enterotoxigenic E. coli (ETEC

-including ETEC:F4, ETEC:F18, ETEC:F5, ETEC:F41, ETEC:F17, etc.

-Shiga toxin-producing E. coli (STEC) or verotoxigenic E. coli (VTEC)

-including enterohemorrhagic E. coli (EHEC) and edema disease E. coli (EDEC)

-Enteropathogenic E. coli (EPEC)

-Extra-intestinal E. coli (ExPEC)

-including avian E. coli (APEC) and uropathogenic E. coli (UPEC)

-Enteroaggregative E. coli (EAEC

-Other specific pathotypes on request (CDEC, DAEC, SEPEC, etc)

 

The EcL Laboratory also has a collection of various E. coli reference and PCR control strains, available upon request.

Each strain is accompanied by a data sheet indicating (when available):

-the virotype *

-serotype (O:H) 

-ST (MLST)

-phylogenetic group

-origin of the strain (species, age of the animal, organ, clinical signs)

-the year of isolation

 

* Subtyping of Stx1 and / or Stx2 is available on request (additional fees) if the information is not already available as in the case of a sequenced strain.

To obtain one or more of these strains, the Université de Montréal requires the signature of a Material Transfer Agreement (MTA), which stipulates that the strain(s) must be used for research purposes only and that they must not be used for any other purpose or transferred to a third party without written authorization from the Université de Montréal.

 

In addition, you will be required to provide proof of compliance of your facilities and operations equivalent to a level 2 containment from your governmental or institutional authority.

 

Finally, you may be required to apply for an import permit if the shipment is international.

 

Rate

A fee of C$175** per strain (to cover preparation costs) is charged. This does not include shipping costs or the cost of applying for an import permit, which are the responsibility of the recipient. A preferential rate may be available for non-profit or academic institutions.

** Prices subject to change without notice ($ CAN)

Please contact ghyslaine.vanier@umontreal.ca  for any request for quotes or information.

If you have specific needs (e.g. virulence genes or resistance profile), we can suggest strains from our collection for you to choose from.